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InitialsDiceBearhttps://github.com/dicebear/dicebearhttps://creativecommons.org/publicdomain/zero/1.0/„Initials” (https://github.com/dicebear/dicebear) by „DiceBear”, licensed under „CC0 1.0” (https://creativecommons.org/publicdomain/zero/1.0/)M
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2 yr. ago

  • Ain't no step about it.

  • Not monkey but rocky mountain oysters.

  • NULL

    Jump
  • My vehicle gets washed when it rains, and I live in the US southwest.

  • In my opinion, no. It is picked and consumed when the buds are still tight, if the head starts to loosen or the buds begin to open it has a more bitter taste to it.

  • Deleted

    Disproven

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  • 5-10 steps ahead of them after they lapped them twice and going for a third. A lot of people just don't seem to understand how much time and passion scientist put into their research.

  • From the research paper (behind the paywall) it appears they only tested cancer cells, shown below, and on mouse models. It's been awhile since I've studied this, so I don't know if the proteins involved are specific to cancer cells or not. If not I'd assume it would kill all cells. With the mouse models I assume they injected directly into the tumor for targeted treatment, but I didn't dive into it that deep.

    Paper link: https://faseb.onlinelibrary.wiley.com/doi/full/10.1096/fj.202500412R?saml_referrer

    2.2 Cell Culture

    Human monocyte-like THP-1 leukemia cells (THP-1, THP-1Asc-KO, THP-1Gsdmd-KO, THP-1-Null, THP-1-defCasp1, and THP-1-defNLRP3) [4, 21] were provided by Professor Li Sun's lab (Institute of Oceanology, Chinese Academy of Sciences, Qingdao, China), and human liver cancer Huh7.5 cells were maintained in our lab. THP-1 cells and Huh7.5 cells were cultured in RPMI 1640 medium with 10% FBS, 100 U/mL penicillin, and 100 μg/mL streptomycin at 37°C in a 5% CO2 humidified incubator. All experiments were carried out with the same batch of cell lines between passages 2 and 8.

    2.15 Xenograft Tumor Mouse Model

    BALB/C nu-nu male mice, 4 weeks old, were obtained from Beijing Vital River Laboratory Animal Technology Co. Ltd. (Beijing, China). A total of 3 × 106 Huh7.5 cells were subcutaneously injected into the right fore flank of each nude mouse. The daily drug treatment began when the tumor size reached ~100 mm3 and continued for a further 2 weeks as follows: EPS3.9 (30 and 60 mg kg−1 d−1, intraperitoneal injection) dissolved in assisted solvent (PBS); Control groups were given the same volume of PBS. Body weight and tumor volumes were measured every day with a balance or with a vernier caliper. The tumor volume was calculated with the formula: 1/2 × [length × (width)2]. After treatment for 2 weeks, mice were sacrificed by decapitation and tumor tissues were collected for further analysis.

  • Don't have open flames when opening that bad boy.

  • Actually an American, a lost treasure, Anthony Bourdain. Loved his shows.

  • blah blah blah....Epstein files

  • How does it go?

    Rules for thee, not for me.

  • A good book on this is: Free Culture: The Nature and Future of Creativity by Lawrence Lessig

  • Sister by another mister.

  • It is what it is.

  • That was definitely not a clean poop.

    video

  • If you take into consideration the self-ionization of water, it's both, at the same time.

    2 H2O -> H3O+ + -OH

  • And they've spent more than that on these shitty ads, I've seen them several times on my local channels.

  • “Trump took in $240 million in bribes under the guise of being for his inauguration his pay to play scheme”